abstract：:The pharmacokinetics of decursin and decursinol angelate (D/DA) was investigated in male SD rats following oral and intravenous administration. D/DA and metabolites obtained from in vitro samples were evaluated by LC/MS. The level of D/DA and metabolized decursinol in the blood following oral and intravenous administr...

journal_title：Journal of microbiology and biotechnology

authors： Kim KM,Kim MJ,Kang JS

更新日期：2009-12-01 00:00:00

abstract：:Small heat shock proteins (sHSPs) function as molecular chaperones that protect cells against environmental stresses. In the present study, the genes of hsp17.6 and hsp17.7, cytosolic class I sHSPs, were cloned from a tropical plant, Ageratina adenophorum. Their C-terminal domains were highly conserved with those of s...

journal_title：Journal of microbiology and biotechnology

authors： Gong W,Yue M,Xie B,Wan F,Guo J

更新日期：2009-12-01 00:00:00

abstract：:The evolution of living organisms occurs via a combination of highly complicated processes that involve modification of various features such as appearance, metabolism and sensing systems. To understand the evolution of life, it is necessary to understand how each biological feature has been optimized in response to n...

journal_title：Journal of microbiology and biotechnology

authors： Nguyen TV,Hong SH,Lee SY

更新日期：2009-11-01 00:00:00

abstract：:Response surface methodology (RSM) examining the effects of five-level-three-factors and their mutual interactions was utilized to optimize the fermentation conditions to enhance capsular polysaccharide (CPS) production of Streptococcus pneumoniae type 3. Twenty experiments conducted in an 8-l lab-scale fermentor were...

journal_title：Journal of microbiology and biotechnology

authors： Jin SD,Kim YM,Kang HK,Jung SJ,Kim D

更新日期：2009-11-01 00:00:00

abstract：:Two novel avian beta-defensins (AvBDs) isolated from duck liver were characterized and their homologies with other AvBDs were analyzed. They were shown to be duck AvBD9 and AvBD10. The mRNA expression of the two genes was analyzed in 17 different tissues from 1-28-dayold ducks. AvBD9 was differentially expressed in th...

journal_title：Journal of microbiology and biotechnology

authors： Ma D,Liao W,Wang R,Han Z,Liu S

更新日期：2009-11-01 00:00:00

abstract：:The bioassay-guided fractionation of MeOH extract from Naematoloma fasciculare afforded a petroleum ether fraction (NFPF) and four known compounds, which showed good antitumor activities to inhibit MCF-7 cell line proliferation in vitro and tumor growth in H(22) implanted mice in vivo. In addition, a number of unsatur...

journal_title：Journal of microbiology and biotechnology

authors： Yan D,Bao HY,Bau T,Li Y,Kim YH

更新日期：2009-10-01 00:00:00

abstract：:Whitening effect, tyrosinase inhibitions, and cytotoxicity of neoagarotetraose were measured after purification from hydrolyzed agar by gel filtration chromatography. In melanoma B16F10 cells, melanin content of neoagarotetraose-treated cells was as same as that treated by kojic acid or arbutin. In addition, tyrosinas...

journal_title：Journal of microbiology and biotechnology

authors： Jang MK,Lee DG,Kim NY,Yu KH,Jang HJ,Lee SW,Jang HJ,Lee YJ,Lee SH

更新日期：2009-10-01 00:00:00

abstract：:The objectives of this study were to determine the antibiotypes, SCCmec subtypes, PVL carriage, and genetic diversity of MRSA strains from a tertiary hospital. Sixtysix MRSA strains were selected randomly (2003, 2004, and 2007) and tested for the Panton-Valentine leukocidin gene, mecA gene, and SCCmec type via a PCR. ...

journal_title：Journal of microbiology and biotechnology

authors： Thong KL,Junnie J,Liew FY,Yusof MY,Hanifah YA

更新日期：2009-10-01 00:00:00

abstract：:The abundance, biomass, size distribution, and taxonomic composition of bacterial and protistan (heterotrophic and autotrophic nanoflagellates and ciliates) communities were investigated in six lakes of Masurian Lake District (north-eastern Poland) differing in trophic state. Samples were taken from the trophogenic wa...

journal_title：Journal of microbiology and biotechnology

authors： Ryszard CJ,Tomasz A,Kalinowska K,Skowronska A

更新日期：2009-09-01 00:00:00

abstract：:Several species of Gram-positive bacteria have cell wall peptidoglycan (syn. murein) in which not all of the sugar moieties are N-acetylated. This has recently been shown to be a secondary effect, caused by the action of a peptidoglycan N-acetylglucosamine deacetylase. We have found that the opportunistic pathogen Lis...

journal_title：Journal of microbiology and biotechnology

authors： Popowska M,Kusio M,Szymanska P,Markiewicz Z

更新日期：2009-09-01 00:00:00

abstract：:The identification and characterization of Bacillus polyfermenticus KJS-2 (B. polyfermenticus KJS-2) was conducted using TEM, an API 50CHB kit, 16S rDNA sequencing, a phylogenetic tree, and catalase and oxidase testing. The conversion rate of glucose to lactic acid by B. polyfermenticus KJS-2 was found to be 60.71+/-4...

journal_title：Journal of microbiology and biotechnology

authors： Kim KM,Kim MJ,Kim DH,Park YS,Kang JS

更新日期：2009-09-01 00:00:00

abstract：:Fungal diversity during composting was investigated by culture-independent rDNA sequence analysis. Composting was carried out with pig manure and mushroom cultural waste using a field-scale composter (Hazaka system), and samples were collected at various stages. Based on partial sequence analysis of large subunit (LSU...

journal_title：Journal of microbiology and biotechnology

authors： Cho KM,Kwon EJ,Kim SK,Kambiranda DM,Math RK,Lee YH,Kim J,Yun HD,Kim H

更新日期：2009-08-01 00:00:00

abstract：:A beta-1,3-1,4-glucanase from the fungus Laetiporus sulphureus var. miniatus was purified as a single 26 kDa band by ammonium sulfate precipitation, HiTrap Q HP, and UNO Q ion-exchange chromatography, with a specific activity of 29 U/mg. The molecular mass of the native enzyme was 52 kDa as a dimer by gel filtration. ...

journal_title：Journal of microbiology and biotechnology

authors： Hong MR,Kim YS,Joo AR,Lee JK,Kim YS,Oh DK

更新日期：2009-08-01 00:00:00

abstract：:Using 200 porcine colon tissues, the efficiencies of three isolation methods of Campylobacter from porcine intestines were compared: Method 1, direct streaking of colon mucosa; Method 2, direct inoculation of intestinal contents with a swab; Method 3, inoculation of pre-enriched medium. A total of 460 Campylobacter is...

journal_title：Journal of microbiology and biotechnology

authors： Shin E,Lee Y

更新日期：2009-07-01 00:00:00

abstract：:The dependence of the catalytic properties of horseradish peroxidase on the structural changes of ionic liquids was investigated with two water-miscible ionic liquids, N-butyl-3- methypyridinium tetrafluoroborate ([BMPy][BF4]) and 1-butyl- 3-methylimidazolium methylsulfate ([BMIM][MeSO4]), each of which shares an anio...

journal_title：Journal of microbiology and biotechnology

authors： Hong ES,Park JH,Yoo IK,Ryu KG

更新日期：2009-07-01 00:00:00

abstract：:Leaves are the major biomass-producing organs in herbaceous plants and mainly develop during vegetative stage by activities of shoot apical meristem. There is a strong correlation between leaf number and bolting, a characteristic phenotype during the transition to reproductive phase in Arabidopsis thaliana. In order t...

journal_title：Journal of microbiology and biotechnology

authors： Lee BH

更新日期：2009-06-01 00:00:00

abstract：:As a provirus, polydnavirus has a segmented DNA genome on chromosome(s) of host wasp. It contains several genes in each segment that presumably play critical roles in regulating physiological processes of target insect parasitized by the wasp. A cysteine-rich protein 1 (CRP1) is present in the polydnavirus Cotesia plu...

journal_title：Journal of microbiology and biotechnology

authors： Barandoc K,Kim Y

更新日期：2009-06-01 00:00:00

abstract：:The biosynthesis study of antibiotics saframycin (SFM) in Streptomyces lavendulae and safracin (SAC) in Pseudomonas fluorescens demonstrated that 3-hydroxy-5-methyl-Omethyltyrosine (3h5mOmTyr), a nonproteinogenic amino acid, is the precursor of the tetrahydroisoquinoline molecular core. In the biosynthetic gene cluste...

journal_title：Journal of microbiology and biotechnology

authors： Fu CY,Tang MC,Peng C,Li L,He YL,Liu W,Tang GL

更新日期：2009-05-01 00:00:00

abstract：:A chimeric gene encoding enhanced green fluorescent protein (EGFP) and a S-layer protein from Lactobacillus brevis KCTC3102, and/or two copies of the Fc-binding Z-domain, a synthetic analog of the B-domain of protein A, was constructed and expressed in Escherichia coli BL21(DE3). The S-layer fusion proteins produced i...

journal_title：Journal of microbiology and biotechnology

authors： Khang YH,Park HY,Jeong YS,Kim JA,Kim YH

更新日期：2009-05-01 00:00:00

abstract：:The purified endochitosanase (Mw 41 kDa) from bacterium Bacillus cereus D-11 hydrolyzed chitooligomers (GlcN)5-7 into chitobiose, chitotriose, and chitotetraose as the final products. The minimal size of the oligosaccharides for enzymatic hydrolysis was a pentamer. To further investigate the cleavage pattern of this e...

journal_title：Journal of microbiology and biotechnology

authors： Gao XA,Jung WJ,Kuk JH,Park RD

更新日期：2009-04-01 00:00:00

abstract：:Hepatitis B core antigen (HBcAg) is an important serological marker used in the diagnosis of hepatitis B virus (HBV) infections. In the current study, a fast and efficient preparative purification protocol for truncated HBcAg from Escherichia coli disruptate was developed. The recombinant HBcAg was first captured by a...

journal_title：Journal of microbiology and biotechnology

authors： Ho CW,Tan WS,Chong FC,Ling TC,Tey BT

更新日期：2009-04-01 00:00:00

abstract：:A beta-agarase gene, agaB34, was functionally cloned from the genomic DNA of a marine bacterium, Agarivorans albus YKW-34. The open reading frame of agaB34 consisted of 1,362 bp encoding 453 amino acids. The deduced amino acid sequence, consisting of a typical N-terminal signal peptide followed by a catalytic domain o...

journal_title：Journal of microbiology and biotechnology

authors： Fu XT,Pan CH,Lin H,Kim SM

更新日期：2009-03-01 00:00:00

abstract：:Interleukin-13 (IL-13) has been proposed as a therapeutic target for bronchial asthma as it plays crucial roles in the pathogenesis of the disease. We developed an in vitro test system measuring transcriptional downregulatory activities on IL-13 as a primary screening method to select drug candidates from natural prod...

journal_title：Journal of microbiology and biotechnology

authors： Choi JJ,Park BK,Park S,Yun CY,Kim DH,Kim JS,Hwang ES,Jin M

更新日期：2009-03-01 00:00:00

abstract：:Acinetobacter baumannii BD5 was isolated from waters of Baek-du mountain, and the lipase gene was cloned using a PCR technique. The deduced amino acid sequence of the lipase and lipase chaperone were found to encode proteins of 325 aa and 344 aa with a molecular mass of 35 kDa and 37 kDa, respectively. The lipase gene...

journal_title：Journal of microbiology and biotechnology

authors： Park IH,Kim SH,Lee YS,Lee SC,Zhou Y,Kim CM,Ahn SC,Choi YL

更新日期：2009-02-01 00:00:00

abstract：:HpaGXooc, from rice pathogenic bacterium Xanthomonas oryzae pv. oryzicola, is a member of the harpin group of proteins, eliciting hypersensitive cell death in non-host plants, inducing disease and insect resistance in plants, and enhancing plant growth. To express and secret the HpaGXooc protein in Bacillus subtilis, ...

journal_title：Journal of microbiology and biotechnology

authors： Wu H,Wang S,Qiao J,Liu J,Zhan J,Gao X

更新日期：2009-02-01 00:00:00

abstract：:Microbial oxidoreductive systems have been widely used in asymmetric syntheses of optically active alcohols. However, when reused in multi-batch reaction, the catalytic efficiency and sustainability of non-growing cells usually decreased because of continuous consumption of required cofactors during the reaction proce...

journal_title：Journal of microbiology and biotechnology

authors： Nie Y,Xu Y,Hu QS,Xiao R

更新日期：2009-01-01 00:00:00

abstract：:Adipose tissue is an important endocrine organ involved in the control of whole body energy homeostasis and insulin sensitivity. Considering the increased incidence of obesity and obesity-related disorders, including diabetes, it is important to understand thoroughly the process of adipocyte differentiation and its co...

journal_title：Journal of microbiology and biotechnology

authors： Atiar Rahman M,Kumar SG,Lee SH,Hwang HS,Kim HA,Yun JW

更新日期：2008-12-01 00:00:00

abstract：:A denitrifying polyphosphate-accumulating bacterium (YKP-9) was isolated from activated sludge of a 5-stage biological nutrient removal process with step feed system. This organism was a Gram-negative, coccus-shaped, facultative aerobic chemoorganotroph. It had a respiratory type of metabolism with oxygen, nitrate, an...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Park YK

更新日期：2008-12-01 00:00:00

abstract：:Effects of various industrially important carbon sources (glucose, sucrose, xylose, gluconate, and glycerol) on shikimic acid (SA) biosynthesis in Escherichia coli were investigated to gain new insight into the metabolic capability for overproducing SA. At the outset, constraints-based flux analysis using the genome-s...

journal_title：Journal of microbiology and biotechnology

authors： Ahn JO,Lee HW,Saha R,Park MS,Jung JK,Lee DY

更新日期：2008-11-01 00:00:00

abstract：:Vibrio vulnificus is a causative agent of serious diseases in humans, resulting from the contact of wound with seawater or consumption of raw seafood. Several studies aimed at detecting V. vulnificus have targeted vvh as a representative virulence toxin gene belonging to the bacterium. In this study, we targeted the r...

journal_title：Journal of microbiology and biotechnology

authors： Kim DG,Ahn SH,Kim LH,Park KJ,Hong YK,Kong IS

更新日期：2008-11-01 00:00:00

abstract：:A novel putative toxin-antitoxin segregational stability system named KyAB system was identified in a novel native plasmid pBMB8240 from Bacillus thuringiensis strain YBT-1520, based on sequences homology with other toxin-antitoxin systems, the lethal activity of the KyB putative toxin in Escherichia coli and the stab...

journal_title：Journal of microbiology and biotechnology

authors： Liu X,Zhu S,Ye W,Ruan L,Yu Z,Zhao C,Sun M

更新日期：2008-10-01 00:00:00

abstract：:Lactobacillus casei 3260 (L. casei 3260) was evaluated in relation to the inflammatory response mediated by lipopolysaccharide (LPS)-induced nuclear factor-kappaB (NF-kappaB) and cyclooxygenase-2 (COX-2) expression in Raw264.7 macrophage cells. The treatment of Raw264.7 cells with L. casei 3260 significantly inhibited...

journal_title：Journal of microbiology and biotechnology

authors： Lee JM,Hwang KT,Jun WJ,Park CS,Lee MY

更新日期：2008-10-01 00:00:00

abstract：:MhMTS and MhMTH are trehalose (alpha-D-glucopyranosyl- [1,1]-alpha-D-glucopyranose) biosynthesis genes of the thermophilic microorganism Metallosphaera hakonensis, and encode a maltooligosyltrehalose synthase (MhMTS) and a maltooligosyltrehalose trehalohydrolase (MhMTH), respectively. In this study, the two genes were...

journal_title：Journal of microbiology and biotechnology

authors： Seo JS,An JH,Cheong JJ,Choi YD,Kim CH

更新日期：2008-09-01 00:00:00

abstract：:It has been reported that Tat-SOD can be directly transduced into mammalian cells and skin and acts as a potential therapeutic protein in various diseases. To isolate the compound that can enhance the transduction efficiency of Tat-SOD, we screened a number of natural products. 3-O-[beta-D-Glucopyranosyl(1-->4)-alpha-...

journal_title：Journal of microbiology and biotechnology

authors： Lee SH,Kim SY,Kim DW,Jang SH,Lim SS,Kwon HJ,Kang TC,Won MH,Kang IJ,Lee KS,Park J,Eum WS,Choi SY

更新日期：2008-09-01 00:00:00

abstract：:The roles of conserved amino acid residues (Val329-Ala330- Asn331-Glu332), constituting an extra sugar-binding space (ESBS) of Thermus maltogenic amylase (ThMA), were investigated by combinatorial saturation mutagenesis. Various ThMA mutants were firstly screened on the basis of starch hydrolyzing activity and their e...

journal_title：Journal of microbiology and biotechnology

authors： Oh SW,Jang MU,Jeong CK,Kang HJ,Park JM,Kim TJ

更新日期：2008-08-01 00:00:00

abstract：:Nitrospira is a dominant member of nitrite-oxidizing bacteria (NOB) in nitrifying bioreactors as well as in natural habitats. In this study, Nitrospira NOB were investigated in the two nitrifying reactors operated with high and low dissolved oxygen (DO) concentrations for a period of 300 days. Phylogenetic and termina...

journal_title：Journal of microbiology and biotechnology

authors： Park HD,Noguera DR

更新日期：2008-08-01 00:00:00

abstract：:Pseudomonas aeruginosa is an opportunistic human pathogen that produces and secretes exopolysaccharides (EPS), in which cells are embedded to form a highly organized community structure called biofilm. Here, we characterized the role of cyclic diguanylate (c-di-GMP) and EPS (PEL) overproduction in the wspF mutant phen...

journal_title：Journal of microbiology and biotechnology

authors： Chung IY,Choi KB,Heo YJ,Cho YH

更新日期：2008-07-01 00:00:00

abstract：:In this study, we investigated the inhibition effects of single and mixed heavy metal ions (Zn2+, Ni2+, Cu2+, and Cd2+) on iron oxidation by Acidithiobacillus ferrooxidans. Effects of metals on the iron oxidation activity of A. ferrooxidans are categorized into four types of patterns according to its oxidation behavio...

journal_title：Journal of microbiology and biotechnology

authors： Cho KS,Ryu HW,Choi HM

更新日期：2008-07-01 00:00:00

abstract：:A Gram-negative, strictly aerobic, motile bacterial strain, designated Gsoil 124T, was isolated from a soil sample taken from a ginseng field in Pocheon Province (South Korea). The isolate contained Q-10 as the predominant lipoquinone, plus C18:1 7c and summed feature 4 (C16:1 6c and/or iso- C15:0 2-OH) as the major f...

journal_title：Journal of microbiology and biotechnology

authors： Lee HW,Ten IL,Jung HM,Liu QM,Im WT,Lee ST

更新日期：2008-06-01 00:00:00

abstract：:Glucagon, a peptide hormone produced by alpha-cells of Langerhans islets, is a physiological antagonist of insulin and stimulator of its secretion. In order to improve its bioactivity, we modified its structure at the C-terminus by amidation catalyzed by a recombinant amidase in bacterial cells. The human gene coding ...

journal_title：Journal of microbiology and biotechnology

authors： Qi X,Jiang R,Yao C,Zhang R,Li Y

更新日期：2008-06-01 00:00:00